ADVES-自然科学教育高度化部門

🧬🐟🌳🐝身近な生命科学実習 2023S

🧬🐟🌳🐝ADVES Boot Camp – Molecular Biology in our daily lives – (2023S)

🧬🐟🌳🐝身近な生命科学実習 2023S

🧬🐟🌳🐝ADVES Boot Camp – Molecular Biology in our daily lives – (2023S)

現在作成中です。シラバス公開までおまちください。

🧬🐟🌳🐝

Experiments a lot & “thinking training”

It is complicated to classify tuna (sushi, fillets, blocks) by taste or shape unless you are a fish expert. So then, how do we determine the type of tuna accurately and reproducibly?

We will first receive a tuna fillet or sushi as a specimen in this practical course. Next, we will analyse the tuna using molecular biological methods to determine its tuna species. Finally, we will give a mini-presentation on our results in this course.

This course aims to learn (1) basic experimental skills and discussion methods and (2) an understanding of life science terms and techniques such as DNA and PCR, often mentioned in the daily news.


Table of Contents

0. For students who are considering taking this course
1. Content of this course
2. Guidance
3. Schedule of the practical course
4. Laboratory
5. Contact information (from students to faculty staff)
6. Class communication (from faculty to students)


0. For students who are considering taking this course

We welcome participation by life science novices and non-science majors from any division.

Only students attending the face-to-face class are eligible to take the course.

If the number of students exceeds the capacity, there will be a selection process for those who can take the course. For details, please check 0-2.

1. Content of this course

Part 1 (A-side)

To Acquire skills that form the basis of molecular biological experiments.

Perform DNA extraction, amplification by PCR, DNA sequencing by Sanger sequencing method, and sequence analysis from tuna fillets or blocks. The final DNA sequence information obtained is compared with known tuna sequences by database reference. Based on the results, students will discuss which species of tuna genus they were given the fillets and present the results discussed in a lab meeting style.

General flow

  • Explanation of the contents of this course
  • DNA extraction from tuna specimen
  • DNA amplification by PCR
  • Electrophoresis of the DNA fragments and detection
  • Sequencing of PCR products by Sanger sequencing method
  • Identification of tuna species based on the sequencing results
  • Presentation and discussion

Part 2 (B-side)

“Thinking training”

In addition to the above flow, individuals/groups are given simple assignments that even beginners can understand, with individualised attention to the participating students’ learning achievement and experimental progress. We cannot easily find the answer by searching the Web or browsing past print collections. Also, each student/group needs to design and conduct various experiments.
“Thinking training” will be added to the work content of the prescribed practical flow, the experience of challenging tasks for which the answers are not fixed. We hope that you will enjoy your stay on this course.

2. Guidance

Guidance will be given individually or in small groups via Zoom. Please get in touch with us at the email address below.

practical-bio@adves.c.u-tokyo.ac.jp

3. Schedule of the practical course

The course will be an intensive lecture in a face-to-face format on the dates and times listed below.

Depending on the progress of the experiment, it may not be possible to complete the course at the following time.

Students who cannot attend the practical training day due to periodic examinations or other individual circumstances should be sure to notify us in advance. Please make individual schedule adjustments so as not to affect the progress of the practical course.

We will update the schedule as soon as it is decided.

4. Laboratory

KOMCEE EAST 3rd floor, Laboratory for Educational Development (EDL)

Google map: https://goo.gl/maps/jceXhmfc1BTot8vj6

5. Contact information (from students to faculty staff)

– ADVES Bootcamp Email:

practical-bio@adves.c.u-tokyo.ac.jp

If you have any questions, please do not hesitate to contact us.

6. Class communication (from faculty to students)

1) Until the number of students who can register for the class is confirmed (until the selection process is completed), the latest information will be sent to NNNN as needed.

2) After the selection process is complete, we will invite only students eligible to take the class to Google Classroom for this class. In addition, we will use our Google Classroom for specific communication about the course distribution of course materials and assignments.

For personal communications that do not need to be shared with the entire class, we will use email (practical-bio@adves.c.u-tokyo.ac.jp).


This page is about the following class.

ADVES Boot Camp -Molecular Biology in our daily lives-
Classification of tuna species using Molecular Biology Approach
Target: Students in any division interested in life science are welcome.
Course Code: 40214
Term/Semester: S2


2023/01/13 ADVES003🐟